Impact of Extraction Solvent On the Antimicrobial Potential of the Root of Verbascum Blattaria

Abstract

Different solvent extracts of the root of Verbascum blataria were screened for antimicrobial activity in the present study. Extracts of the root were prepared in various solvents and tested against fungal and bacterial species (Klebsiella pneumoniae, Bacillus atrophaeus Pseudomonas aeruginosa, Citrobacter freundi, Salmonella typhi, Agrobacterium tumefaciens, Alternaria solani, Aspergillus fumigatus, Candida albicane,). Antifungal and antibacterial assays were performed by well diffusion and disc diffusion methods. The screening of the different solvent-extracted samples revealed that methanol extract was more effective against B.subtillus, B.atrophus, A. tumefaciens, C. freundi, K. pneumoniae, A.solani, and A. fumigatus. Methanol extract formed the maximum zone of inhibition of 67% ZI at 3000µg/disc against B.atrophus than other tested microorganisms.  The hexane extract produced a maximum zone of inhibition against B.subtillus (32% ZI), E.coli (54 % ZI), and A. tumefaciens (52% ZI) while the ethyl acetate extract reduced the growth of P. aerugenosa with high potential than other extracts.