Improvement Of Bacillus Subtilis Natto Viability By Alginate And Xanthan Gum As A Wall Material

Abstract

In this study, Bacillus subtilis natto was encapsulated by alginate with (coated or mixed) or without xanthan gum as a supplement component and evaluated for survival in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). The results showed that B. sublitis natto biomass had a thrombolytic ability compared to the control sample. The viability of encapsulated B. subtilis natto was improved, and alginate 2% (w/v) had a high encapsulation efficiency. There was no difference between the samples with or without the xanthan gum supplement. In SGF and SIF tests, the B. subtilis viability in samples supplemented with xanthan gum was higher than that of the samples using only alginate, and there was no significant difference between containing xanthan gum mixing or coating. The results indicated that adding xanthan gum is necessary to increase alginate's protective effect on B. subtilis natto